Difference between revisions of "Part:BBa K3889010"
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<partinfo>BBa_K3889010 short</partinfo> | <partinfo>BBa_K3889010 short</partinfo> | ||
| − | This is a synthetically derived promoter sequence for | + | This is a synthetically derived promoter sequence for constitutive expression of genes in <i>Bacillus subtilis</i>. |
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!Promoter | !Promoter | ||
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[[Media:T--IISER-Tirupati_India--Synthetic_promoter_of_Bacillus_subtilis.pdf|Synthetic Promoters data from SP1-214]][1] | [[Media:T--IISER-Tirupati_India--Synthetic_promoter_of_Bacillus_subtilis.pdf|Synthetic Promoters data from SP1-214]][1] | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>) | + | SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>) and SP200(<partinfo>BBa_K3889012</partinfo>) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. The library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences: |
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Based on this the promoters are as follows: | Based on this the promoters are as follows: | ||
| − | [[File:T--IISER-Tirupati India--SP allignment.png]] | + | [[File:T--IISER-Tirupati India--SP allignment.png]]<br> |
| − | + | Fig source: Geneious version 2021.2 created by Biomatters. Available from https://www.geneious.com | |
===Modelling=== | ===Modelling=== | ||
| − | We | + | We planned to use SP126 with <partinfo>BBa_K1351033</partinfo> for gene expression of SRTF1 (<partinfo>BBa_K3889100</partinfo>). |
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[[File:T--IISER-Tirupati India--SP126.jpg|900 px]] | [[File:T--IISER-Tirupati India--SP126.jpg|900 px]] | ||
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<partinfo>BBa_K3889010 parameters</partinfo> | <partinfo>BBa_K3889010 parameters</partinfo> | ||
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===References=== | ===References=== | ||
1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115 | 1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115 | ||
Latest revision as of 20:15, 18 October 2021
Bacillus Subtilis Synthetic Promoter 126
This is a synthetically derived promoter sequence for constitutive expression of genes in Bacillus subtilis.
| Promoter | Sequence 5‘ → 3‘ | Relative activity wrt P43 of GFP (%) | Standard deviation |
|---|---|---|---|
| SP126 | AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT | 29.07 | 0.23 |
| SP146 | AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT | 40.39 | 0.69 |
| SP200 | AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA | 76.82 | 0.9 |
Synthetic Promoters data from SP1-214[1]
Usage and Biology
SP126(BBa_K3889010), SP146(BBa_K3889011) and SP200(BBa_K3889012) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. The library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:
Based on this the promoters are as follows:
Fig source: Geneious version 2021.2 created by Biomatters. Available from https://www.geneious.com
Modelling
We planned to use SP126 with BBa_K1351033 for gene expression of SRTF1 (BBa_K3889100).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115