Difference between revisions of "Part:BBa K3889012"
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This is a synthetically derived promoter sequence for the expression of genes in <i>Bacillus subtilis</i>.
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This is a synthetically derived promoter sequence for constitutive expression of genes in <i>Bacillus subtilis</i>.
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{| class=wikitable style="text-align: center;"
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<body>
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|-
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<table style="height: 297px;" width="864" border="1">
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!Promoter
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<body>
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!Sequence 5‘ → 3‘
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<tr>
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!Relative activity wrt P43 of GFP (%)
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<td style="width: 79.7812px;">
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!Standard deviation
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<p><strong>Promoter</strong></p>
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|-
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</td>
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|SP126
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<td style="width: 522.781px;">
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|AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT
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<p><strong>Sequence 5&lsquo; &#8594; 3&lsquo;</strong></p>
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|29.07
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</td>
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|0.23
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<td style="width: 154.812px;">
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|-
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<p><strong>Relative</strong></p>
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|SP146
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<p><strong>activity wrt P43 of</strong></p>
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|AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT
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<p><strong>GFP (%)</strong></p>
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|40.39
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</td>
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|0.69
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<td style="width: 78.625px;">
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|-
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<p><strong>Standard</strong></p>
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|SP200
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<p><strong>deviation</strong></p>
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|AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA
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</td>
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|76.82
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</tr>
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|0.9
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<tr>
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|}
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<td style="width: 79.7812px;">
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<p><span style="font-weight: 400;">SP126</span></p>
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</td>
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<td style="width: 522.781px;">
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<p><span style="font-weight: 400;">AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT</span></p>
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</td>
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<td style="width: 154.812px;">
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<p><span style="font-weight: 400;">29.07</span></p>
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</td>
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<td style="width: 78.625px;">
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<p><span style="font-weight: 400;">0.23</span></p>
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</td>
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</tr>
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<tr>
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<td style="width: 79.7812px;">
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<p><span style="font-weight: 400;">SP146</span></p>
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</td>
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<td style="width: 522.781px;">
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<p><span style="font-weight: 400;">AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT</span></p>
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</td>
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<td style="width: 154.812px;">
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<p><span style="font-weight: 400;">40.39</span></p>
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</td>
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<td style="width: 78.625px;">
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<p><span style="font-weight: 400;">0.69</span></p>
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</td>
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</tr>
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<tr>
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<td style="width: 79.7812px;">
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<p><span style="font-weight: 400;">SP200</span></p>
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</td>
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<td style="width: 522.781px;">
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<p><span style="font-weight: 400;">AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA</span></p>
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</td>
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<td style="width: 154.812px;">
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<p><span style="font-weight: 400;">76.82</span></p>
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</td>
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<td style="width: 78.625px;">
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<p><span style="font-weight: 400;">0.9</span></p>
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</td>
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</tr>
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</tbody>
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</table>
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</body>
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</html>
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[[Media:T--IISER-Tirupati_India--Synthetic_promoter_of_Bacillus_subtilis.pdf|Synthetic Promoters data from SP1-214]][1]
 
[[Media:T--IISER-Tirupati_India--Synthetic_promoter_of_Bacillus_subtilis.pdf|Synthetic Promoters data from SP1-214]][1]
  
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===Modelling===
 
===Modelling===
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We planned to use SP200 with <partinfo>BBa_K1351031</partinfo> for gene expression of mf-Lon (<partinfo>BBa_K3889101</partinfo>).
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We planned to use SP200 with <partinfo>BBa_K1351031</partinfo> for gene expression of P22(<partinfo>BBa_K3889101</partinfo>).
 
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<body>
 
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Revision as of 04:27, 17 October 2021


Bacillus subtilis Synthetic Promoter 200


This is a synthetically derived promoter sequence for constitutive expression of genes in Bacillus subtilis.

Promoter Sequence 5‘ → 3‘ Relative activity wrt P43 of GFP (%) Standard deviation
SP126 AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT 29.07 0.23
SP146 AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT 40.39 0.69
SP200 AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA 76.82 0.9

Synthetic Promoters data from SP1-214[1]


Usage and Biology

SP126(BBa_K3889010), SP146(BBa_K3889011), SP200(BBa_K3889010) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. Since the library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:


Based on this the promoters are as follows: T--IISER-Tirupati India--SP allignment.png


Modelling

We planned to use SP200 with BBa_K1351031 for gene expression of P22(BBa_K3889101).

Parameters used:

Rate of P22 production = 9218880/nM*day

Rate of P22 degradation = 1.69/day

T--IISER-Tirupati India--SP200.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115