Difference between revisions of "Part:BBa K3889011"

 
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<partinfo>BBa_K3889011 short</partinfo>
 
<partinfo>BBa_K3889011 short</partinfo>
  
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Synthetically designed bacillus promoter
 
  
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This is a synthetically derived promoter sequence for the expression of genes in <i>Bacillus subtilis</i>.
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<html>
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<body>
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<table style="height: 297px;" width="864" border="1">
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<body>
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<tr>
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<td style="width: 79.7812px;">
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<p><strong>Promoter</strong></p>
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</td>
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<td style="width: 522.781px;">
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<p><strong>Sequence 5&lsquo; &#8594; 3&lsquo;</strong></p>
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</td>
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<td style="width: 154.812px;">
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<p><strong>Relative</strong></p>
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<p><strong>activity wrt P43 of</strong></p>
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<p><strong>GFP (%)</strong></p>
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</td>
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<td style="width: 78.625px;">
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<p><strong>Standard</strong></p>
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<p><strong>deviation</strong></p>
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</td>
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</tr>
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<tr>
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<td style="width: 79.7812px;">
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<p><span style="font-weight: 400;">SP126</span></p>
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</td>
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<td style="width: 522.781px;">
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<p><span style="font-weight: 400;">AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT</span></p>
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</td>
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<td style="width: 154.812px;">
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<p><span style="font-weight: 400;">29.07</span></p>
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</td>
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<td style="width: 78.625px;">
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<p><span style="font-weight: 400;">0.23</span></p>
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</td>
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</tr>
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<tr>
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<td style="width: 79.7812px;">
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<p><span style="font-weight: 400;">SP146</span></p>
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</td>
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<td style="width: 522.781px;">
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<p><span style="font-weight: 400;">AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT</span></p>
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</td>
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<td style="width: 154.812px;">
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<p><span style="font-weight: 400;">40.39</span></p>
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</td>
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<td style="width: 78.625px;">
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<p><span style="font-weight: 400;">0.69</span></p>
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</td>
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</tr>
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<tr>
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<td style="width: 79.7812px;">
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<p><span style="font-weight: 400;">SP200</span></p>
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</td>
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<td style="width: 522.781px;">
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<p><span style="font-weight: 400;">AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA</span></p>
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</td>
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<td style="width: 154.812px;">
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<p><span style="font-weight: 400;">76.82</span></p>
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</td>
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<td style="width: 78.625px;">
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<p><span style="font-weight: 400;">0.9</span></p>
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</td>
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</tr>
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</tbody>
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</table>
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</body>
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</html>
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[[Media:T--IISER-Tirupati_India--Synthetic_promoter_of_Bacillus_subtilis.pdf|Synthetic Promoters data from SP1-214]][1]
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===Usage and Biology===
 
===Usage and Biology===
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SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>), SP200(<partinfo>BBa_K3889010</partinfo>) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. Since the library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:
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<html>
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<head>
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<style>
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div.scroll {overflow-x: aut0; overflow-y: hidden;}
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</style>
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</head>
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<body>
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<div class="scroll">
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<img src="https://static.igem.org/mediawiki/parts/8/88/T--IISER-Tirupati_India--SP_backbone.jpeg">
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</div>
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</body>
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</html>
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Based on this the promoters are as follows:
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[[File:T--IISER-Tirupati India--SP allignment.png]]
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===Modelling===
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We used SP146 with <partinfo>BBa_K1351030</partinfo> for gene expression of mf-Lon (<partinfo>BBa_K3889122</partinfo>).
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<html>
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<body>
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<p><b>Parameters used:</b></p>
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<p>Rate of mf-Lon production  = 5.7885 x 10<sup>7</sup>/day</p>
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<p>Rate of mf-Lon degradation = 1.69/day</p>
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</body>
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</html>
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[[File:T--IISER-Tirupati India--SP146.jpg|900 px]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3889011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3889011 SequenceAndFeatures</partinfo>

Revision as of 17:34, 16 October 2021


Bacillus subtilis Synthetic Promoter 146


This is a synthetically derived promoter sequence for the expression of genes in Bacillus subtilis.

Promoter

Sequence 5‘ → 3‘

Relative

activity wrt P43 of

GFP (%)

Standard

deviation

SP126

AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT

29.07

0.23

SP146

AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT

40.39

0.69

SP200

AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA

76.82

0.9
Synthetic Promoters data from SP1-214[1]


Usage and Biology

SP126(BBa_K3889010), SP146(BBa_K3889011), SP200(BBa_K3889010) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. Since the library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:


Based on this the promoters are as follows: T--IISER-Tirupati India--SP allignment.png


Modelling

We used SP146 with BBa_K1351030 for gene expression of mf-Lon (BBa_K3889122).

Parameters used:

Rate of mf-Lon production = 5.7885 x 107/day

Rate of mf-Lon degradation = 1.69/day

T--IISER-Tirupati India--SP146.jpg



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]