Difference between revisions of "Protein domains/Overview"

Revision as of 01:37, 27 March 2009

Protein tags and modifiers are short peptide sequences cloned in frame with protein coding sequences that change the protein's behavior.

Protein tags and modifiers might

• change the protein's location (localization signals)
• enable it to be readily purified (affinity tags)
• fuse two protein domains together (linkers)
• alter the protein's degradation rate (degradation tags)
• target the protein for cleavage (cleavage sites)

Protein tags may include a start codon or a stop codon, but not both, since they are intended to be assembled in frame with protein coding sequences (?).