Difference between revisions of "Part:BBa K4047005"
 
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===Long Description===
 
===Long Description===
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This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal+fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.
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This is the forward primer pairing with the reverse primer BBa_K4047004, to amplify the gene for fructose-1,6-bisphosphatase. This allows the insertion of both fructose-1,6-bisphosphatase and transaldolase into the pGEM-tal+fbp plasmid (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone (BBa_K4047024). The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification.
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This primer is entirely BioBrick compatible, making it readily useable for future iGEM teams.

Latest revision as of 04:14, 16 October 2021


primerTF_fbp-foward

Forward primer for FBPase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Long Description

This is the forward primer pairing with the reverse primer BBa_K4047004, to amplify the gene for fructose-1,6-bisphosphatase. This allows the insertion of both fructose-1,6-bisphosphatase and transaldolase into the pGEM-tal+fbp plasmid (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone (BBa_K4047024). The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification.

This primer is entirely BioBrick compatible, making it readily useable for future iGEM teams.