Difference between revisions of "Part:BBa K4047002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This primer was created using the information detailed below within the NEBuilder free online tool located at https://nebuilder.neb.com/#!/ | |
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Overlap sequence sourced from pAM2991. | Overlap sequence sourced from pAM2991. | ||
https://www.addgene.org/40248/ | https://www.addgene.org/40248/ | ||
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Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase class I, at the locus tag Synpcc7942_2335. | Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase class I, at the locus tag Synpcc7942_2335. | ||
https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2402921&to=2403955&report=fasta&strand=2 | https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2402921&to=2403955&report=fasta&strand=2 | ||
===References=== | ===References=== | ||
Latest revision as of 04:09, 16 October 2021
primerF_fbp-foward
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 20
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 20
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 20
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 20
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 20
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This primer was created using the information detailed below within the NEBuilder free online tool located at https://nebuilder.neb.com/#!/
Source
Generated from a combination of factors. Overlap sequence sourced from pAM2991. https://www.addgene.org/40248/
Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase class I, at the locus tag Synpcc7942_2335. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2402921&to=2403955&report=fasta&strand=2