Difference between revisions of "Part:BBa K4047004"
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===Long Description===
 
===Long Description===
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This is the reverse primer to amplify the gene for transaldolase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal+fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.
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This is the reverse primer (pairing with the forward primer BBa_K4047005) to amplify and allow insertion of transaldolase and fructose-1,6-bisphosphatase into pGEM-tal+ fbp (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone BBa_K4047024. The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification.
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This primer does not contain any BioBrick incompatible sequences, making it a very useful option for teams that want to amplify transaldolase from the wild type genome.

Revision as of 04:04, 16 October 2021


primerTF_tal-reverse

Rev primer for transadolase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Long Description

This is the reverse primer (pairing with the forward primer BBa_K4047005) to amplify and allow insertion of transaldolase and fructose-1,6-bisphosphatase into pGEM-tal+ fbp (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone BBa_K4047024. The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification.

This primer does not contain any BioBrick incompatible sequences, making it a very useful option for teams that want to amplify transaldolase from the wild type genome.