Difference between revisions of "Part:BBa K3889010"
Line 6: Line 6:
 
<html>
 
<html>
 
<body>
 
<body>
−
<table style="height: 297px;" width="864">
+
<table style="height: 297px;" width="864" border="1">
 
<body>
 
<body>
 
<tr>
 
<tr>
Line 13: Line 13:
 
</td>
 
</td>
 
<td style="width: 522.781px;">
 
<td style="width: 522.781px;">
−
<p><strong>Sequence 5&lsquo; 3&lsquo;</strong></p>
+
<p><strong>Sequence 5&lsquo; &#8594; 3&lsquo;</strong></p>
 
</td>
 
</td>
 
<td style="width: 154.812px;">
 
<td style="width: 154.812px;">
Line 77: Line 77:
 
SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>), SP200(<partinfo>BBa_K3889010</partinfo>) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. Since the library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:
 
SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>), SP200(<partinfo>BBa_K3889010</partinfo>) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. Since the library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:
 
<html>
 
<html>
 +
<head>
 +
<style>
 +
div.scroll {overflow-x: aut0; overflow-y: hidden;}
 +
</style>
 +
</head>
 
<body>
 
<body>
−
<marquee behavior="scroll" direction="left">
+
<div class="scroll">
 
<img src="https://static.igem.org/mediawiki/parts/8/88/T--IISER-Tirupati_India--SP_backbone.jpeg">
 
<img src="https://static.igem.org/mediawiki/parts/8/88/T--IISER-Tirupati_India--SP_backbone.jpeg">
−
</marquee>
+
</div>
 
</body>
 
</body>
 
</html>
 
</html>

Revision as of 03:46, 11 October 2021


Bacillus Subtilis Synthetic Promoter 126

This is a synthetically derived promoter sequence for the expression of genes in Bacillus subtilis.

Promoter

Sequence 5‘ → 3‘

Relative

activity wrt P43 of

GFP (%)

Standard

deviation

SP126

AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT

29.07

0.23

SP146

AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT

40.39

0.69

SP200

AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA

76.82

0.9
Synthetic Promoters data from SP1-214[1]


Usage and Biology

SP126(BBa_K3889010), SP146(BBa_K3889011), SP200(BBa_K3889010) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. Since the library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115