Difference between revisions of "Part:BBa K3889010"

Revision as of 18:13, 8 October 2021

Bacillus Subtilis Synthetic Promoter 126

This is a synthetically derived promoter sequence for the expression of genes in Bacillus subtilis.

Promotor	Sequence 5‘ 3‘	Relative activity wrt P43 of GFP (%)	Standard deviation
SP126	AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT	29.07	0.23
SP146	AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT	40.39	0.69
SP200	AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA	76.82	0.9

Synthetic Promoters data from SP1-214[1]

Usage and Biology

SP126(BBa_K3889010), SP146(BBa_K3889011), SP200(BBa_K3889010) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are constitutive hence can be used in general protein production. Since the library contains 214 synthetic promoters providing with variability in expression according to the system need. All SP promoters have the following consensus sequences:

T--IISER-Tirupati India--SP backbone.jpeg

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 75: / Line 75: @@
 ===Usage and Biology===
-SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>), SP200(<partinfo>BBa_K3889010</partinfo>) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are constitutive hence can be used in general protein production. Since the library contains 214 synthetic promoters providing with variability in expression according to the system need.
+SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>), SP200(<partinfo>BBa_K3889010</partinfo>) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are constitutive hence can be used in general protein production. Since the library contains 214 synthetic promoters providing with variability in expression according to the system need. All SP promoters have the following consensus sequences:
-All SP promoters has these consensus sequences:
 [[File:T--IISER-Tirupati India--SP backbone.jpeg|900px|left]]