Difference between revisions of "Part:BBa K3889010"

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This is a synthetically derived promoter sequence for the expression of genes in <i>Bacillus subtilis</i>.
 
This is a synthetically derived promoter sequence for the expression of genes in <i>Bacillus subtilis</i>.
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<html>
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<body>
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<table style="height: 297px;" width="864">
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<tbody>
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<tr>
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<td style="width: 79.7812px;">
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<p><strong>Promotor</strong></p>
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</td>
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<td style="width: 522.781px;">
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<p><strong>Sequence 5&lsquo; 3&lsquo;</strong></p>
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</td>
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<td style="width: 154.812px;">
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<p><strong>Relative</strong></p>
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<p><strong>activity wrt P43 of</strong></p>
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<p><strong>GFP (%)</strong></p>
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</td>
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<td style="width: 78.625px;">
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<p><strong>Standard</strong></p>
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<p><strong>deviation</strong></p>
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</td>
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</tr>
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<tr>
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<td style="width: 79.7812px;">
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<p><span style="font-weight: 400;">SP126</span></p>
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</td>
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<td style="width: 522.781px;">
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<p><span style="font-weight: 400;">AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT</span></p>
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</td>
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<td style="width: 154.812px;">
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<p><span style="font-weight: 400;">29.07</span></p>
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</td>
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<td style="width: 78.625px;">
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<p><span style="font-weight: 400;">0.23</span></p>
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</td>
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</tr>
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<tr>
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<td style="width: 79.7812px;">
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<p><span style="font-weight: 400;">SP146</span></p>
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</td>
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<td style="width: 522.781px;">
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<p><span style="font-weight: 400;">AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT</span></p>
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</td>
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<td style="width: 154.812px;">
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<p><span style="font-weight: 400;">40.39</span></p>
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</td>
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<td style="width: 78.625px;">
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<p><span style="font-weight: 400;">0.69</span></p>
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</td>
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</tr>
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<tr>
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<td style="width: 79.7812px;">
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<p><span style="font-weight: 400;">SP200</span></p>
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</td>
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<td style="width: 522.781px;">
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<p><span style="font-weight: 400;">AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA</span></p>
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</td>
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<td style="width: 154.812px;">
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<p><span style="font-weight: 400;">76.82</span></p>
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</td>
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<td style="width: 78.625px;">
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<p><span style="font-weight: 400;">0.9</span></p>
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</td>
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</tr>
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</tbody>
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</table>
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</body>
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</html>
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[[Media:T--IISER-Tirupati_India--Synthetic_promoter_of_Bacillus_subtilis.pdf|Synthetic Promoters data from SP1-214]][1]
  
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[[Media:T--IISER-Tirupati_India--Synthetic_promoter_of_Bacillus_subtilis.pdf]]
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===Usage and Biology===
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SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>), SP200(<partinfo>BBa_K3889010</partinfo>) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are constitutive hence can be used in general protein production. Since the library contains 214 synthetic promoters providing with variability in expression according to the system need.
  
  

Revision as of 09:17, 7 October 2021


Bacillus Subtilis Synthetic Promoter 126

This is a synthetically derived promoter sequence for the expression of genes in Bacillus subtilis.

Promotor

Sequence 5‘ 3‘

Relative

activity wrt P43 of

GFP (%)

Standard

deviation

SP126

AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT

29.07

0.23

SP146

AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT

40.39

0.69

SP200

AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA

76.82

0.9
Synthetic Promoters data from SP1-214[1]


Usage and Biology

SP126(BBa_K3889010), SP146(BBa_K3889011), SP200(BBa_K3889010) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are constitutive hence can be used in general protein production. Since the library contains 214 synthetic promoters providing with variability in expression according to the system need.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]