Difference between revisions of "Part:BBa K4047014"

 
 
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<partinfo>BBa_K4047014 parameters</partinfo>
 
<partinfo>BBa_K4047014 parameters</partinfo>
 
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===Long Description===
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This is the reverse primer to amplify the downstream (3’) portion of the gene glpX encoding sedoheptulose- 1,7-bisphosphatase (Synpcc7942_0505). The overlap sequence (red) allows proper Gibson assembly with the pGEM-SBPase backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification. The amplicon would be used for the composite part pGEM-SBPase.

Latest revision as of 18:42, 4 October 2021


dSBPase_glpX_DS-rev

Reverse glpX_DS for SBPase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Long Description

This is the reverse primer to amplify the downstream (3’) portion of the gene glpX encoding sedoheptulose- 1,7-bisphosphatase (Synpcc7942_0505). The overlap sequence (red) allows proper Gibson assembly with the pGEM-SBPase backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification. The amplicon would be used for the composite part pGEM-SBPase.