Difference between revisions of "Part:BBa K4047003:Design"
Anniequyan (Talk | contribs) (→Source) |
|||
Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
+ | Generated from a combination of factors. | ||
+ | Overlap sequence sourced from pAM2991. | ||
+ | https://www.addgene.org/40248/ | ||
− | + | Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase class I, at the locus tag Synpcc7942_2335. | |
− | + | https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2402921&to=2403955&report=fasta&strand=2 | |
===References=== | ===References=== |
Revision as of 18:10, 4 October 2021
primerF/TF_fbp-reverse
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 14
Illegal PstI site found at 2 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 20
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 14
Illegal PstI site found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 14
Illegal PstI site found at 2 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
Generated from a combination of factors. Overlap sequence sourced from pAM2991. https://www.addgene.org/40248/
Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase class I, at the locus tag Synpcc7942_2335. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2402921&to=2403955&report=fasta&strand=2