Difference between revisions of "Protein domains/Overview"
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*target the protein for cleavage (cleavage sites)
 
*target the protein for cleavage (cleavage sites)
  
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Protein tags may include a start codon or a stop codon, but not both, since they are intended to be assembled in frame with protein coding sequences.
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Protein tags may include a start codon or a stop codon, but not both, since they are intended to be assembled in frame with '''[[Protein coding sequences|protein coding sequences]] [[Help:Protein coding sequences|(?)]]'''.
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'''You may also be interested in'''
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{|
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|valign='top' align='center' width=50px | {{Click || image=Part icon cds.png | link=Protein coding sequences |width=30px | height=30px}}
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|valign='top' |'''[[Protein coding sequences]] [[Help:Protein coding sequences|(?)]]''': Protein coding sequences encode the amino acid sequence of a particular protein. Note that some protein coding sequences only encode a protein domain or half a protein.  Others encode a full-length protein from start codon to stop codon.
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Revision as of 01:06, 22 January 2009

Protein tags and modifiers are short peptide sequences cloned in frame with protein coding sequences that change the protein's behavior.

Protein tags and modifiers might

  • change the protein's location (localization signals)
  • enable it to be readily purified (affinity tags)
  • fuse two protein domains together (linkers)
  • alter the protein's degradation rate (degradation tags)
  • target the protein for cleavage (cleavage sites)

Protein tags may include a start codon or a stop codon, but not both, since they are intended to be assembled in frame with protein coding sequences (?).