Difference between revisions of "Protein domains/Overview"
| Line 4: | Line 4: | ||
*change the protein's location (localization signals) | *change the protein's location (localization signals) | ||
*enable it to be readily purified (affinity tags) | *enable it to be readily purified (affinity tags) | ||
| + | *fuse two protein domains together (linkers) | ||
*alter the protein's degradation rate (degradation tags) | *alter the protein's degradation rate (degradation tags) | ||
*target the protein for cleavage (cleavage sites) | *target the protein for cleavage (cleavage sites) | ||
Revision as of 23:13, 11 January 2009
Protein tags and modifiers are short peptide sequences cloned in frame with protein coding sequences that change the protein's behavior.
Protein tags and modifiers might
- change the protein's location (localization signals)
- enable it to be readily purified (affinity tags)
- fuse two protein domains together (linkers)
- alter the protein's degradation rate (degradation tags)
- target the protein for cleavage (cleavage sites)
Protein tags may include a start codon or a stop codon, but not both, since they are intended to be assembled in frame with protein coding sequences.
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| Protein coding sequences (?): Protein coding sequences encode the amino acid sequence of a particular protein. Note that some protein coding sequences only encode a protein domain or half a protein. Others encode a full-length protein from start codon to stop codon. |
