Difference between revisions of "Part:BBa K3804008:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
No special design consideration.
+
We used tctagaGAAAGAGGGGACAAactag in BBa_J61100 as RBS and deleted 6bp(taataa) which locate in the end of LasI. Thus, the length of this sequence is 792.
  
  
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===Source===
 
===Source===
  
−
The part consists of sigma70pro, RBS, LasI and terminator
+
The part consists of sigma70promoter(<bbpart>BBa_J23100</bbpart>), RBS(<bbpart>BBa_J61100</bbpart>), LasI(<bbpart>BBa_C0178</bbpart>) and double terminator(<bbpart>BBa_B0015</bbpart>)
  
 
===References===
 
===References===

Latest revision as of 05:49, 30 September 2021


LasI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 270
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used tctagaGAAAGAGGGGACAAactag in BBa_J61100 as RBS and deleted 6bp(taataa) which locate in the end of LasI. Thus, the length of this sequence is 792.


Source

The part consists of sigma70promoter(BBa_J23100), RBS(BBa_J61100), LasI(BBa_C0178) and double terminator(BBa_B0015)

References