Difference between revisions of "Part:BBa I732955"
 
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[[Image:USTC_plasmid_955.jpg]]
 
[[Image:USTC_plasmid_955.jpg]]
  
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*'''[[User:Rshetty|RS]] 01:32, 14 September 2008 (UTC)''': Note that this plasmid has been split into a plasmid backbone and part.  The plasmid backbone is BBa_I732950 and the part is I732827.  The physical DNA associated with this part has sequence BBa_I732950-I732827. ''This part needs to be deleted and the physical DNA remapped to the correct part number.''
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{{:Template:Partplasmidsplit}}
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The original part is now BBa_I732950-I732827.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:41, 24 November 2008

Ptemplate1 + RBS.ARL2A0103.TERM

Genotype Character Name
Top10/pT-TERM So not express any repressors CR00
Top10/pT-ARL4A0604 Constitutively express ARL4A0604 CR01
Top10/pT-ARL2A0203 Constitutively express ARL2A0203 CR10
Top10/pTet-ARL4A0604-ARL2A203 Constitutively express ARL4A0604 and ARL2A0203 CR11

USTC plasmid 955.jpg

  • RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.

The original part is now BBa_I732950-I732827.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1242
    Illegal EcoRI site found at 4409
    Illegal XbaI site found at 4574
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4409
    Illegal SpeI site found at 1243
    Illegal PstI site found at 1257
    Illegal NotI site found at 1250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4409
    Illegal XhoI site found at 2276
    Illegal XhoI site found at 3302
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1243
    Illegal EcoRI site found at 4409
    Illegal XbaI site found at 4574
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4409
    Illegal XbaI site found at 4574
    Illegal SpeI site found at 1243
    Illegal PstI site found at 1257
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3448
    Illegal BsaI.rc site found at 4433