Difference between revisions of "Part:BBa K3779004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | gaattcgcggccgcttctagtactagaggaacaaaaactcatctcagaagaggatctgtactagagcatcatcatcatcatcattactagaggtttgtagccttagacatgactgttcctcagttcaagttgggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatttgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtataggattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgcagctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttcttggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggatcctactagagcccacacaccatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaattttccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttgaaatttttttttttagtttttttctctttcagtgacctccattgatatttaagttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgttcattagaaagaaagcatagcaatctaatctaaggggctactagaggtgttgacaattaatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaacctactagatggccaagttgaccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggggagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgttgccgaggagcaggactgatactagagcacgtccgacggcggcccacgggtcccaggcctcggagatccgtcccccttttcctttgtcgatatcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcaagcttactagatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggtactagaggactggttccaattgacaagctactagatgagatttccttcaatttttactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacagaagatgaaacggcacaaattccggctgaagctgtcatcggttactcagatttagaaggggatttcgatgttgctgttttgccattttccaacagcacaaataacgggttattgtttataaatactactattgccagcattgctaaagaagaaggggtatctctcgagaagagagaggctgaacgatactagagtattcttttccaaattcttttagattcggttggtctcaagctggttttcaatctgagatgggtactccaggatctgaagatccaaatactgattggtataagtgggttcatgacccagagaacatggctgctggtttggtttctggtgacttaccagaaaacggtcctggttactggggtaactacaagacttttcatgataacgctcaaaagatgggtttgaaaatcgctagattgaacgtcgaatggtctagaatctttcctaatccactaccaagacctcaaaattttgatgagtctaaacaagatgttactgaagttgaaattaatgagaatgaattgaaaagacttgatgaatacgctaacaaggatgcattgaaccactatagagaaatttttaaggatttgaagtctagaggattgtattttattttgaatatgtaccattggccattgcctttgtggttgcacgatccaattagagttagaagaggtgactttactggtccatctggatggctttctactagaaccgtttacgagtttgctagattttcagcttacattgcttggaaatttgatgatttggttgatgaatactctactatgaacgaacctaatgttgttggtggtttaggttatgttggagttaaatctggttttcctccaggatatttgtccttcgaattgtctagaagagctatgtataatattattcaagcacatgctagagcttatgacggtattaagtctgtttcaaagaaacctgttggaattatctacgctaattcatcttttcaacctttgaccgataaagacatggaagctgttgaaatggctgaaaacgataatagatggtggtttttcgatgctattattagaggtgagattaccagaggtaatgaaaagattgttagagatgacttgaagggtaggttggactggatcggtgttaattactacactagaactgttgttaagagaactgaaaagggttacgtgtcattaggaggttatggtcatggttgtgaaaggaactctgtttctttggctggtctgccaacttctgattttggttgggaattctttccagagggtttgtacgatgtgttgactaaatattggaacaggtatcatttgtacatgtatgttactgaaaacggtattgctgacgatgctgattaccaacgtccatactaccttgtttctcacgtttatcaagttcatagagctattaattcaggtgctgatgttagaggttacttgcattggtcattggctgataactacgaatgggcttctggattttctatgagatttggtttgttgaaggttgattataacactaaaagattatattggagacctagtgctttagtttacagagaaattgctactaacggagctataactgatgaaattgaacatttgaattcagttccaccagttaagccattgagacattaatactagtagcggccgctgcag | |
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===Source=== | ===Source=== | ||
Latest revision as of 17:52, 27 September 2021
pPICZαA
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1257
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BamHI site found at 1677
Illegal XhoI site found at 1184
Illegal XhoI site found at 1246 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1224
Illegal NgoMIV site found at 2435
Illegal NgoMIV site found at 2496
Illegal AgeI site found at 1396 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1660
Illegal BsaI.rc site found at 1859
Illegal BsaI.rc site found at 2857
Design Notes
gaattcgcggccgcttctagtactagaggaacaaaaactcatctcagaagaggatctgtactagagcatcatcatcatcatcattactagaggtttgtagccttagacatgactgttcctcagttcaagttgggcacttacgagaagaccggtcttgctagattctaatcaagaggatgtcagaatgccatttgcctgagagatgcaggcttcatttttgatacttttttatttgtaacctatatagtataggattttttttgtcattttgtttcttctcgtacgagcttgctcctgatcagcctatctcgcagctgatgaatatcttgtggtaggggtttgggaaaatcattcgagtttgatgtttttcttggtatttcccactcctcttcagagtacagaagattaagtgagaccttcgtttgtgcggatcctactagagcccacacaccatagcttcaaaatgtttctactccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaaattttccctctttcttcctctagggtgtcgttaattacccgtactaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatcacgtttctttttcttgaaatttttttttttagtttttttctctttcagtgacctccattgatatttaagttaataaacggtcttcaatttctcaagtttcagtttcatttttcttgttctattacaactttttttacttcttgttcattagaaagaaagcatagcaatctaatctaaggggctactagaggtgttgacaattaatcatcggcatagtatatcggcatagtataatacgacaaggtgaggaactaaacctactagatggccaagttgaccagtgccgttccggtgctcaccgcgcgcgacgtcgccggagcggtcgagttctggaccgaccggctcgggttctcccgggacttcgtggaggacgacttcgccggtgtggtccgggacgacgtgaccctgttcatcagcgcggtccaggaccaggtggtgccggacaacaccctggcctgggtgtgggtgcgcggcctggacgagctgtacgccgagtggtcggaggtcgtgtccacgaacttccgggacgcctccgggccggccatgaccgagatcggggagcagccgtgggggcgggagttcgccctgcgcgacccggccggcaactgcgtgcacttcgttgccgaggagcaggactgatactagagcacgtccgacggcggcccacgggtcccaggcctcggagatccgtcccccttttcctttgtcgatatcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcaagcttactagatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggtactagaggactggttccaattgacaagctactagatgagatttccttcaatttttactgctgttttattcgcagcatcctccgcattagctgctccagtcaacactacaacagaagatgaaacggcacaaattccggctgaagctgtcatcggttactcagatttagaaggggatttcgatgttgctgttttgccattttccaacagcacaaataacgggttattgtttataaatactactattgccagcattgctaaagaagaaggggtatctctcgagaagagagaggctgaacgatactagagtattcttttccaaattcttttagattcggttggtctcaagctggttttcaatctgagatgggtactccaggatctgaagatccaaatactgattggtataagtgggttcatgacccagagaacatggctgctggtttggtttctggtgacttaccagaaaacggtcctggttactggggtaactacaagacttttcatgataacgctcaaaagatgggtttgaaaatcgctagattgaacgtcgaatggtctagaatctttcctaatccactaccaagacctcaaaattttgatgagtctaaacaagatgttactgaagttgaaattaatgagaatgaattgaaaagacttgatgaatacgctaacaaggatgcattgaaccactatagagaaatttttaaggatttgaagtctagaggattgtattttattttgaatatgtaccattggccattgcctttgtggttgcacgatccaattagagttagaagaggtgactttactggtccatctggatggctttctactagaaccgtttacgagtttgctagattttcagcttacattgcttggaaatttgatgatttggttgatgaatactctactatgaacgaacctaatgttgttggtggtttaggttatgttggagttaaatctggttttcctccaggatatttgtccttcgaattgtctagaagagctatgtataatattattcaagcacatgctagagcttatgacggtattaagtctgtttcaaagaaacctgttggaattatctacgctaattcatcttttcaacctttgaccgataaagacatggaagctgttgaaatggctgaaaacgataatagatggtggtttttcgatgctattattagaggtgagattaccagaggtaatgaaaagattgttagagatgacttgaagggtaggttggactggatcggtgttaattactacactagaactgttgttaagagaactgaaaagggttacgtgtcattaggaggttatggtcatggttgtgaaaggaactctgtttctttggctggtctgccaacttctgattttggttgggaattctttccagagggtttgtacgatgtgttgactaaatattggaacaggtatcatttgtacatgtatgttactgaaaacggtattgctgacgatgctgattaccaacgtccatactaccttgtttctcacgtttatcaagttcatagagctattaattcaggtgctgatgttagaggttacttgcattggtcattggctgataactacgaatgggcttctggattttctatgagatttggtttgttgaaggttgattataacactaaaagattatattggagacctagtgctttagtttacagagaaattgctactaacggagctataactgatgaaattgaacatttgaattcagttccaccagttaagccattgagacattaatactagtagcggccgctgcag
Source
Commercial synthetic