Difference between revisions of "Protein domains/Overview"
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*alter the protein's degradation rate (degradation tags) | *alter the protein's degradation rate (degradation tags) | ||
*target the protein for cleavage (cleavage sites) | *target the protein for cleavage (cleavage sites) | ||
+ | |||
+ | Protein tags may include a start codon or a stop codon, but not both, since they are intended to be assembled in frame with protein coding sequences. |
Revision as of 02:55, 17 November 2008
Protein tags and modifiers are short peptide sequences cloned in frame with protein coding sequences that change the protein's behavior.
Protein tags and modifiers might
- change the protein's location (localization signals)
- enable it to be readily purified (affinity tags)
- alter the protein's degradation rate (degradation tags)
- target the protein for cleavage (cleavage sites)
Protein tags may include a start codon or a stop codon, but not both, since they are intended to be assembled in frame with protein coding sequences.