Difference between revisions of "Part:BBa K3411010"
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Lambert_GA iGEM 2020's NarP system is modeled after <i>E.coli’s</i> natural nitrite and nitrate sensor: the Nar Operon, which includes the NarP response regulator, the gene listed above, and NarQ, the membrane-bound sensor protein. The NarP/NarQ system detects both nitrite and nitrate. This specific part is the isolated NarP gene. | Lambert_GA iGEM 2020's NarP system is modeled after <i>E.coli’s</i> natural nitrite and nitrate sensor: the Nar Operon, which includes the NarP response regulator, the gene listed above, and NarQ, the membrane-bound sensor protein. The NarP/NarQ system detects both nitrite and nitrate. This specific part is the isolated NarP gene. | ||
| − | The listed NarP’s role within Lambert_GA iGEM 2020's NarP System,[https://parts.igem.org/Part:BBa_K3411020 BBa_K3411020], is controlled by the promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106], a constitutive Anderson Promoter, which initiates the production of TetR. TetR represses the PtetO promoter, producing NarP. In the presence of both nitrate and nitrite, NarQ phosphorylates NarP. Therefore, phosphorylated NarP measures both nitrite and nitrate levels. By using the corrected difference, | + | The listed NarP’s role within Lambert_GA iGEM 2020's NarP System,[https://parts.igem.org/Part:BBa_K3411020 BBa_K3411020], is controlled by the promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106], a constitutive Anderson Promoter, which initiates the production of TetR. TetR represses the PtetO promoter, producing NarP. In the presence of both nitrate and nitrite, NarQ phosphorylates NarP. Therefore, phosphorylated NarP measures both nitrite and nitrate levels. By using the corrected difference, the NarP and NarL (Lambert_GA iGEM 2020's improved part of HKUST-Rice iGEM 2015 [https://parts.igem.org/Part:BBa_K1682018 Part:BBa_K1682018] composite parts are able to evaluate the nitrite levels. |
| − | Lambert_GA iGEM 2020 plans on cloning and characterizing | + | Lambert_GA iGEM 2020 plans on cloning and characterizing the NarP part in the coming year as a part of the two-year project, AgroSense. |
Latest revision as of 02:44, 28 October 2020
NarP Gene
Lambert_GA iGEM 2020's NarP system is modeled after E.coli’s natural nitrite and nitrate sensor: the Nar Operon, which includes the NarP response regulator, the gene listed above, and NarQ, the membrane-bound sensor protein. The NarP/NarQ system detects both nitrite and nitrate. This specific part is the isolated NarP gene.
The listed NarP’s role within Lambert_GA iGEM 2020's NarP System,BBa_K3411020, is controlled by the promoter BBa_J23106, a constitutive Anderson Promoter, which initiates the production of TetR. TetR represses the PtetO promoter, producing NarP. In the presence of both nitrate and nitrite, NarQ phosphorylates NarP. Therefore, phosphorylated NarP measures both nitrite and nitrate levels. By using the corrected difference, the NarP and NarL (Lambert_GA iGEM 2020's improved part of HKUST-Rice iGEM 2015 Part:BBa_K1682018 composite parts are able to evaluate the nitrite levels.
Lambert_GA iGEM 2020 plans on cloning and characterizing the NarP part in the coming year as a part of the two-year project, AgroSense.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]