Difference between revisions of "Part:BBa K3411010"

Latest revision as of 02:44, 28 October 2020

NarP Gene

Lambert_GA iGEM 2020's NarP system is modeled after E.coli’s natural nitrite and nitrate sensor: the Nar Operon, which includes the NarP response regulator, the gene listed above, and NarQ, the membrane-bound sensor protein. The NarP/NarQ system detects both nitrite and nitrate. This specific part is the isolated NarP gene.

The listed NarP’s role within Lambert_GA iGEM 2020's NarP System,BBa_K3411020, is controlled by the promoter BBa_J23106, a constitutive Anderson Promoter, which initiates the production of TetR. TetR represses the PtetO promoter, producing NarP. In the presence of both nitrate and nitrite, NarQ phosphorylates NarP. Therefore, phosphorylated NarP measures both nitrite and nitrate levels. By using the corrected difference, the NarP and NarL (Lambert_GA iGEM 2020's improved part of HKUST-Rice iGEM 2015 Part:BBa_K1682018 composite parts are able to evaluate the nitrite levels.

Lambert_GA iGEM 2020 plans on cloning and characterizing the NarP part in the coming year as a part of the two-year project, AgroSense.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3411010 short</partinfo>
-NarP is a part of the Nar Operon which detects both nitrate and nitrite. Our system is modeled after E.coli’s natural nitrite sensor. Similar to NarL system, the promoter BBa_J23106, a constitutive Anderson Promoter, produces TetR, which represses the PtetO promoter, producing NarP. The NarP gene is phosphorylated in the presence of both nitrate and nitrite. Therefore, when NarP eventually phosphorylates PdcuS, which is further explained in the NarP composite Part:BBa_K3411020, it is measuring the levels of both nitrate and nitrite. By using the corrected difference our NarP and NarL composite parts, we are able to evaluate the levels of Nitrite. This specific part is the isolated NarP gene.
+Lambert_GA iGEM 2020's NarP system is modeled after <i>E.coli’s</i> natural nitrite and nitrate sensor: the Nar Operon, which includes the NarP response regulator, the gene listed above, and NarQ, the membrane-bound sensor protein. The NarP/NarQ system detects both nitrite and nitrate. This specific part is the isolated NarP gene.
+The listed NarP’s role within Lambert_GA iGEM 2020's NarP System,[https://parts.igem.org/Part:BBa_K3411020 BBa_K3411020], is controlled by the promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106], a constitutive Anderson Promoter, which initiates the production of TetR. TetR represses the PtetO promoter, producing NarP. In the presence of both nitrate and nitrite, NarQ phosphorylates NarP. Therefore, phosphorylated NarP measures both nitrite and nitrate levels. By using the corrected difference, the NarP and NarL (Lambert_GA iGEM 2020's improved part of HKUST-Rice iGEM 2015 [https://parts.igem.org/Part:BBa_K1682018 Part:BBa_K1682018] composite parts are able to evaluate the nitrite levels.
+Lambert_GA iGEM 2020 plans on cloning and characterizing the NarP part in the coming year as a part of the two-year project, AgroSense.
-Lambert iGEM plans on further characterizing our part in the coming year as a part of our two year project, AgroSense.
 <!-- Add more about the biology of this part here