Difference between revisions of "Part:BBa K3657033"

Latest revision as of 02:22, 28 October 2020

RNA AUAGAU binding Pumby Protein coupled with N-terminal GFP'

This is a fusion protein containing AUAGAU binding Pumby Protein (BBa_K3657004) and N-terminal Split-GFP (BBa_K3657027).

Usage and Biology

AUAGAU binding Pumby Protein binds specifically the AUAGAU RNA sequence(BBa_K3657015). Pumby Proteins can be designed to bind a specific RNA sequence.

N-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of C-terminal Split-GFP (BBa_K3657028).

This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: BBa_K3657036. The part with the C-terminal of the Split-GFP planned for the experiment: BBa_K3657034. You can find out more about the planned experiment here: iGEM Heidelberg 2020.

Due to the COVID19 pandemics we did not obtain empirical data about this part.

Sequence and Features