Difference between revisions of "Part:BBa K3657030"

 
 
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This is a fusion protein containing Antitermination protein N (<a href="https://parts.igem.org/Part:BBa_K3657014">BBa_K3657014</a>) and N-terminal Split-GFP (<a href="https://parts.igem.org/Part:BBa_K3657027">BBa_K3657027</a>).
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===Usage and Biology===
 
===Usage and Biology===
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<p>Antitermination protein N binds specifically the BoxB RNA (<a href="https://parts.igem.org/Part:BBa_K3657015">BBa_K3657015</a>).</p>
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<p>N-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of C-terminal Split-GFP (<a href="https://parts.igem.org/Part:BBa_K3657028">BBa_K3657028</a>).</p>
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<p>This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: <a href="https://parts.igem.org/Part:BBa_K3657036">BBa_K3657036</a>. The part with the C-terminal of the Split-GFP planned for the experiment: <a href="https://parts.igem.org/Part:BBa_K3657031">BBa_K3657031</a>. You can find out more about the planned experiment here: <a href="https://2020.igem.org/Team:Heidelberg/RNA_Protein">iGEM Heidelberg 2020.</a></p>
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<p> Due to the COVID19 pandemics we did not obtain empirical data about this part.</p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3657030 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3657030 SequenceAndFeatures</partinfo>

Latest revision as of 02:19, 28 October 2020


Antitermination protein N coupled with N terminal split GFP

This is a fusion protein containing Antitermination protein N (BBa_K3657014) and N-terminal Split-GFP (BBa_K3657027).

Usage and Biology

Antitermination protein N binds specifically the BoxB RNA (BBa_K3657015).

N-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of C-terminal Split-GFP (BBa_K3657028).


This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: BBa_K3657036. The part with the C-terminal of the Split-GFP planned for the experiment: BBa_K3657031. You can find out more about the planned experiment here: iGEM Heidelberg 2020.

Due to the COVID19 pandemics we did not obtain empirical data about this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]