Difference between revisions of "Part:BBa K3505005"

(→‎Design Notes)
 
(2 intermediate revisions by the same user not shown)
Line 4: Line 4:
  
  
−
TetR- tetR
+
TetR- tet repressor
−
[[Help:Protein coding|Coding region]]  for Tet repressor without an [[Help:Ribosome Binding Site|RBS]] .
+
<p>[[Help:Protein coding|Coding region]]  for Tet repressor without an [[Help:Ribosome Binding Site|RBS]] .</p>
  
  
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
TetR is the repressor of the tetracycline resistance element and a well-characterized transcriptional factor.
  
 
===Design Notes===
 
===Design Notes===

Latest revision as of 00:43, 28 October 2020


TetR- Tet repressor GB compatible with B3-B5


TetR- tet repressor

Coding region for Tet repressor without an RBS .


Usage and Biology

TetR is the repressor of the tetracycline resistance element and a well-characterized transcriptional factor.

Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. This has position B3-B5.

Figure 1.The overhangs of this part in the GoldenBraid Grammar.
Figure 2. The level 0 module : pupd2- TetR (illustration from SnapGene)

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with : SpeI + EcoRI (C2 - C4) , Expected bands : 2047 + 680 bp Positive result : C4

Source

Synthesized by IDT.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]