Difference between revisions of "Part:BBa K3505005"

 
 
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<partinfo>BBa_K3505005 short</partinfo>
 
<partinfo>BBa_K3505005 short</partinfo>
  
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TetR- tetR
 
  
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<!-- Add more about the biology of this part here
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TetR- tet repressor
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<p>[[Help:Protein coding|Coding region]]  for Tet repressor without an [[Help:Ribosome Binding Site|RBS]] .</p>
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===Usage and Biology===
 
===Usage and Biology===
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TetR is the repressor of the tetracycline resistance element and a well-characterized transcriptional factor.
  
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===Design Notes===
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<span class='h3bb'>Sequence and Features</span>
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The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for GoldenBraid cloning. This has position B3-B5.
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<partinfo>BBa_K3505005 SequenceAndFeatures</partinfo>
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<p style="text-align: center;">
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    [[Image: T--Thessaly--GB-AATG-GCTT.jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the GoldenBraid Grammar.</i>]]
  
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[[Image:T--Thessaly--tetR-snap.png |900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- TetR (illustration from SnapGene)</i>]]
  
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<!-- Uncomment this to enable Functional Parameter display
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===Verification of Cloning===
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===Functional Parameters===
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[[File:T--Thessaly--tetr-level0-digestion.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with :
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<partinfo>BBa_K3505005 parameters</partinfo>
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SpeI + EcoRI (C2 - C4) , Expected bands : 2047 + 680 bp
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Positive result : C4 </i>]]
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===Source===
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Synthesized by IDT.
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===Sequence and Features===
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<partinfo>BBa_K3505005 SequenceAndFeatures</partinfo>

Latest revision as of 00:43, 28 October 2020


TetR- Tet repressor GB compatible with B3-B5


TetR- tet repressor

Coding region for Tet repressor without an RBS .


Usage and Biology

TetR is the repressor of the tetracycline resistance element and a well-characterized transcriptional factor.

Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. This has position B3-B5.

Figure 1.The overhangs of this part in the GoldenBraid Grammar.
Figure 2. The level 0 module : pupd2- TetR (illustration from SnapGene)

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with : SpeI + EcoRI (C2 - C4) , Expected bands : 2047 + 680 bp Positive result : C4

Source

Synthesized by IDT.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]