Difference between revisions of "Part:BBa K3425054"
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Asembled using <a href="https://parts.igem.org/Help:Assembly/Scars">Type IIS iGEM Loop assembly standard</a>from the constitutive promoter, BBa_J23100, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425031, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, followed by the double terminator BBa_B0015.
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Asembled using <a href="https://parts.igem.org/Help:Assembly/Scars">Type IIS iGEM Loop assembly standard</a> from the constitutive promoter, BBa_J23100, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425031, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.
 
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Revision as of 00:04, 28 October 2020


Constitutive expression construct of Juniper GFP

Asembled using Type IIS iGEM Loop assembly standard from the constitutive promoter, BBa_J23100, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425031, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 476
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 476
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 476
    Illegal BamHI site found at 685
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 476
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 476
  • 1000
    COMPATIBLE WITH RFC[1000]