Difference between revisions of "Part:BBa K3505005"

Revision as of 21:42, 27 October 2020

TetR- Tet repressor GB compatible with B3-B5

TetR- tetR Coding region for Tet repressor without an RBS .

Usage and Biology

TetR is the repressor of the tetracycline resistance element and a well-characterized transcriptional factor.

Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. This has position B3-B5.

Figure 1.The overhangs of this part in the GoldenBraid Grammar.

Figure 2. The level 0 module : pupd2- TetR (illustration from SnapGene)

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with : SpeI + EcoRI (C2 - C4) , Expected bands : 2047 + 680 bp Positive result : C4

Source

Synthesized by IDT.

Sequence and Features