Difference between revisions of "Part:BBa K3505005"
(→‎Usage and Biology)
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     [[Image: T--Thessaly--GB-AATG-GCTT.jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the Golden Braid Grammar.</i>]]
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     [[Image: T--Thessaly--GB-AATG-GCTT.jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the GoldenBraid Grammar.</i>]]
  
 
[[Image:T--Thessaly--tetR-snap.png |900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- TetR (illustration from SnapGene)</i>]]
 
[[Image:T--Thessaly--tetR-snap.png |900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- TetR (illustration from SnapGene)</i>]]
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===Verification of Cloning===
 
===Verification of Cloning===

Revision as of 20:27, 27 October 2020


TetR- Tet repressor GB compatible with B3-B5


TetR- tetR Coding region for Tet repressor without an RBS .


Usage and Biology

Figure 1.The overhangs of this part in the GoldenBraid Grammar.
Figure 2. The level 0 module : pupd2- TetR (illustration from SnapGene)

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with : SpeI + EcoRI (C2 - C4) , Expected bands : 2047 + 680 bp Positive result : C4

Source

Synthesized by IDT.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]