Difference between revisions of "Part:BBa K143053"

 
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__NOTOC__
 
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<partinfo>BBa_K143053 short</partinfo>
 
<partinfo>BBa_K143053 short</partinfo>
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Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''.
 
Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''.
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Pveg-spoVG can be used in the context of a '''Ribosomes per second''' (RiPS) output generator
 
Pveg-spoVG can be used in the context of a '''Ribosomes per second''' (RiPS) output generator
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'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''
 
'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''
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<!-- Add more about the biology of this part here
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><big>'''Sequence and Features'''</big></span>
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<partinfo>BBa_K143053 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143053 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K143053 parameters</partinfo>
 
<partinfo>BBa_K143053 parameters</partinfo>
 
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<span class='h3bb'><big>'''Part Characterisation'''</big></span>
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Please see the testing constructs [https://parts.igem.org/Part:BBa_K143079 BBa_K143079] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K143082 BBa_K143082] for characterisation data.
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===References===
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<biblio>
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#1 https://parts.igem.org/Assembly:RBS-CDS_issues
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</biblio>

Latest revision as of 22:23, 29 October 2008

Promoter Pveg and RBS spoVG for B. subtilis


Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.


Pveg-spoVG can be used in the context of a Ribosomes per second (RiPS) output generator


To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Part Characterisation

Please see the testing constructs BBa_K143079 and BBa_K143082 for characterisation data.

References

<biblio>

  1. 1 https://parts.igem.org/Assembly:RBS-CDS_issues

</biblio>