Difference between revisions of "Part:BBa K157040:Design"

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===References===
 
===References===
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W. Bruening, B. Giasson, W. Mushynski1 and H. D. Durham (1998): Activation of stress-activated MAP protein kinases up-regulates expression of transgenes driven by the cytomegalovirus immediate/early promoter, in Nucleic Acids Research, 1998, Vol. 26, No. 2

Revision as of 21:10, 29 October 2008

Eukaryotic terminator vector (very similar to pSB1AK8)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 8
    Illegal NotI site found at 3411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XhoI site found at 1272
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2444


Design Notes

CMV Promotor is a viral regulatory element to mediate strong expression of the downstream gene sequence. CMV stands for cytomegalovirus. Because of its strong activity in most cells its a very popular choice to mediate expression of a foreign protein.

Source

The Transfectionvector is according to the BioBrick 2007 part BBa_J52017.
CMV promotor was obtained by PCR by using the Biobrick BBa_J52038 as template.

References

W. Bruening, B. Giasson, W. Mushynski1 and H. D. Durham (1998): Activation of stress-activated MAP protein kinases up-regulates expression of transgenes driven by the cytomegalovirus immediate/early promoter, in Nucleic Acids Research, 1998, Vol. 26, No. 2