Difference between revisions of "Part:BBa K103019:Experience"
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K103019=== | ===Applications of BBa_K103019=== | ||
| + | |||
| + | ==='Hunter and prey' selection system=== | ||
| + | This part was used as core of prey generator device. It was fused with: | ||
| + | *protein A [[Part:BBa_K103003 | BBa_K103003]]. Resulting fusion was named His_alpha_A | ||
| + | *protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. Resulting fusion was named His_alpha_Z | ||
| + | |||
| + | Both fusions were overexpressed in Roseta strain. Due to ability of protein Z to form aggregates His_alpha_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. | ||
| + | His_alpha_A was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_August_2008&arg1=1_September_2008&arg2=2_September_2008&arg3=3_September_2008&arg4=4_September_2008&arg5=5_September_2008&arg6=8_September_2008&arg7=9_September_2008&name=Purification%20of%20proteins%3A%20A-alpha purified] on NiNTA-agarose bead (elution peak in 150 mM imidazole). | ||
| + | The both prey protein fusions were added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]]. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 17:56, 28 October 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K103019
'Hunter and prey' selection system
This part was used as core of prey generator device. It was fused with:
- protein A BBa_K103003. Resulting fusion was named His_alpha_A
- protein ZSPA-1 BBa_K103004. Resulting fusion was named His_alpha_Z
Both fusions were overexpressed in Roseta strain. Due to ability of protein Z to form aggregates His_alpha_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. His_alpha_A was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_August_2008&arg1=1_September_2008&arg2=2_September_2008&arg3=3_September_2008&arg4=4_September_2008&arg5=5_September_2008&arg6=8_September_2008&arg7=9_September_2008&name=Purification%20of%20proteins%3A%20A-alpha purified] on NiNTA-agarose bead (elution peak in 150 mM imidazole). The both prey protein fusions were added to culture media and used in selection experiments. Details here and here.
User Reviews
UNIQf3d6d2ac63e13ef4-partinfo-00000000-QINU UNIQf3d6d2ac63e13ef4-partinfo-00000001-QINU