Difference between revisions of "Part:BBa K157040:Design"
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===Design Notes=== | ===Design Notes=== | ||
The Transfectionvector is according to the BioBrick 2007 part [https://parts.igem.org/Part:BBa_J52017 BBa_J52017].<br> | The Transfectionvector is according to the BioBrick 2007 part [https://parts.igem.org/Part:BBa_J52017 BBa_J52017].<br> | ||
| − | CMV promotor was obtained by PCR | + | CMV promotor was obtained by PCR by using the Biobrick [https://parts.igem.org/Part:BBa_J52038 BBa_J52038] template<br> |
===Source=== | ===Source=== | ||
Revision as of 15:24, 28 October 2008
Eukaryotic terminator vector (very similar to pSB1AK8)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 8
Illegal NotI site found at 3411 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XhoI site found at 1272
Illegal XhoI site found at 2298 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3405
Plasmid lacks a suffix.
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2444
Design Notes
The Transfectionvector is according to the BioBrick 2007 part BBa_J52017.
CMV promotor was obtained by PCR by using the Biobrick BBa_J52038 template
Source
CMV Promotor is a viral regulatory element to mediate strong expression of the downstream genesequence. CMV stands for Cauliflower Mosaic Virus.