Difference between revisions of "Part:BBa K103020:Experience"
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This part was used as core of prey generator device. It was fused with protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. Resulting fusion was named His_omega_Z | This part was used as core of prey generator device. It was fused with protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. Resulting fusion was named His_omega_Z | ||
| − | The fusion was overexpressed in | + | The fusion was overexpressed in Rosetta strain. Due to ability of protein Z to form aggregates His_omega_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. |
The fusion was added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]]. | The fusion was added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]]. | ||
Latest revision as of 12:47, 28 October 2008
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K103020
'Hunter and prey' selection system
This part was used as core of prey generator device. It was fused with protein ZSPA-1 BBa_K103004. Resulting fusion was named His_omega_Z
The fusion was overexpressed in Rosetta strain. Due to ability of protein Z to form aggregates His_omega_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. The fusion was added to culture media and used in selection experiments. Details here and here.
User Reviews
UNIQ5a41d23614a0f10c-partinfo-00000000-QINU UNIQ5a41d23614a0f10c-partinfo-00000001-QINU