Difference between revisions of "Part:BBa K103020:Experience"
 
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==='Hunter and prey' selection system===
 
==='Hunter and prey' selection system===
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This part was used as core of prey generator device. It was fused with:
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This part was used as core of prey generator device. It was fused with protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. Resulting fusion was named His_omega_Z
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*protein A [[Part:BBa_K103003 | BBa_K103003]]. Resulting fusion was named His_omega_A
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*protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. . Resulting fusion was named His_omega_Z
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Both fusions were overexpressed in BL21 strain. Due to ability of protein Z to form aggregates His_omega_Z was purified simply by collecting post-sonication debris.
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The fusion was overexpressed in Rosetta strain. Due to ability of protein Z to form aggregates His_omega_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris.
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His_omega_A was purified on NiNTA-agarose bead (elution peak in 150 mM imidazole).
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The fusion was added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]].
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The both prey protein fusions were added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]].
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===User Reviews===
 
===User Reviews===

Latest revision as of 12:47, 28 October 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K103020

'Hunter and prey' selection system

This part was used as core of prey generator device. It was fused with protein ZSPA-1 BBa_K103004. Resulting fusion was named His_omega_Z

The fusion was overexpressed in Rosetta strain. Due to ability of protein Z to form aggregates His_omega_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. The fusion was added to culture media and used in selection experiments. Details here and here.

User Reviews

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