Difference between revisions of "Part:BBa K3610002:Design"

 
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===Design Notes===
 
===Design Notes===
−
The signal sequence was not cleaved off in-frame. Further it does not contain a start codon, it therefore can not be expressed without linking it to other parts which do.
+
The signal sequence of the full length receptor ([[Part:BBa_K3610001]]) was not cleaved off in-frame. Further it does not contain a start codon, it therefore can not be expressed without linking it to other parts which do.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
  
−
PCR amplified from the BAK1 sequence.
+
Cyril Zipfel Lab at UZH, Switzerland
  
 
===References===
 
===References===
 +
Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.
 +
 +
Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.
 +
 +
Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.
 +
 +
Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.

Latest revision as of 12:59, 3 October 2020


BAK1 from Arabidopsis thaliana without signal sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The signal sequence of the full length receptor (Part:BBa_K3610001) was not cleaved off in-frame. Further it does not contain a start codon, it therefore can not be expressed without linking it to other parts which do.

Source

Cyril Zipfel Lab at UZH, Switzerland

References

Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.

Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.

Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.

Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.