Difference between revisions of "Part:BBa K581008"
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<partinfo>BBa_K581008 short</partinfo> | <partinfo>BBa_K581008 short</partinfo> | ||
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+ | {{Curation/ccdb}} | ||
This is the cell death protein coding sequence under the control of a relatively weak RBS. It is replicable in non-ccdB resistant strains. | This is the cell death protein coding sequence under the control of a relatively weak RBS. It is replicable in non-ccdB resistant strains. | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K581008 parameters</partinfo> | <partinfo>BBa_K581008 parameters</partinfo> | ||
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+ | ==Functional Parameters: Austin_UTexas== | ||
+ | <html> | ||
+ | <body> | ||
+ | <h3><center>Burden Imposed by this Part:</center></h3> | ||
+ | <figure> | ||
+ | <div class = "center"> | ||
+ | <center><img src = "https://static.igem.org/mediawiki/parts/4/43/T--Austin_Utexas--Low_significant_burden.png" style = "width:200px;height:120px"></center> | ||
+ | </div> | ||
+ | <figcaption><center><b>Burden Value: 7.7 ± 4.9% </b></center></figcaption> | ||
+ | </figure> | ||
+ | <p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the | ||
+ | <a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the <a href="https://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team.</a></p> | ||
+ | <p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p> | ||
+ | </p> | ||
+ | </body> | ||
+ | </html> |
Latest revision as of 00:23, 4 September 2020
RBS+ccdB+Termintor
This is the cell death protein coding sequence under the control of a relatively weak RBS. It is replicable in non-ccdB resistant strains.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 233
Functional Parameters
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
![](https://static.igem.org/mediawiki/parts/4/43/T--Austin_Utexas--Low_significant_burden.png)
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.