Difference between revisions of "Part:BBa K733001"
 
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<partinfo>BBa_K733001 short</partinfo>
 
<partinfo>BBa_K733001 short</partinfo>
  
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pTms is a constitutive promoter that can be recognized by SigA factor in Bacillus subtilis. Due to E. coli’s promiscuousness in recognizing transcription and translation signals, pTms is also functional in E. coli. According to some experiments, pTms shows quite low efficiency of transcription in B. subtilis. ( Moran et al., 1982).
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<i>Ptms</i> is a constitutive promoter that can be recognized by SigA factor in ''Bacillus subtilis''. Due to ''E. coli''’s promiscuousness in recognizing transcription and translation signals, <i>Ptms</i> is also functional in ''E. coli''. According to some experiments, <i>Ptms</i> shows quite low efficiency of transcription in ''B. subtilis''. ( Moran et al., 1982).
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For the characterization of <i>Ptms</i>, please refer to: [https://parts.igem.org/Part:BBa_K733009 BBa_K733009]
  
  
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<body>
 
<partinfo>BBa_K733001 parameters</partinfo>
 
<partinfo>BBa_K733001 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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<figcaption><center><b>Burden Value: 6.3 ± 10.6% </b></center></figcaption>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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== '''Reference''' ==
 
== '''Reference''' ==
  
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Moran, C., Lang, N., LeGrice, S., Lee, G., Stephens, M., Sonenshein, A., et al. (1982). Nucleotide sequences that signal the initiation of transcription and translation inBacillus subtilis..Molecular and General Genetics MGG,186, 339-346.
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Moran, C., Lang, N., LeGrice, S., Lee, G., Stephens, M., Sonenshein, A., et al. (1982). Nucleotide sequences that signal the initiation of transcription and translation inBacillus subtilis..''Molecular and General Genetics MGG,186,'' 339-346.

Latest revision as of 22:41, 3 September 2020

Ptms - a constitutive promoter in E. coli and B. subtilis.

Ptms is a constitutive promoter that can be recognized by SigA factor in Bacillus subtilis. Due to E. coli’s promiscuousness in recognizing transcription and translation signals, Ptms is also functional in E. coli. According to some experiments, Ptms shows quite low efficiency of transcription in B. subtilis. ( Moran et al., 1982).

For the characterization of Ptms, please refer to: BBa_K733009


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




Functional Parameters: Austin_UTexas

BBa_K733001 parameters

Burden Imposed by this Part:

Burden Value: 6.3 ± 10.6%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.



Reference

Moran, C., Lang, N., LeGrice, S., Lee, G., Stephens, M., Sonenshein, A., et al. (1982). Nucleotide sequences that signal the initiation of transcription and translation inBacillus subtilis..Molecular and General Genetics MGG,186, 339-346.