Difference between revisions of "Part:BBa C0050"
 
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Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022      pR regulator (BBa_R0050). It represses transcription of the protein encoded      by the sequence 3' to the pR region. This coding sequence does not contain      a RBS.
 
Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022      pR regulator (BBa_R0050). It represses transcription of the protein encoded      by the sequence 3' to the pR region. This coding sequence does not contain      a RBS.
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===Usage and Biology===
 
===Usage and Biology===
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Here is an alignment of the HK022 cI repressor, the lambda cI repressor, the      P22 c2 repressor and the 434 cI repressor in msf format <a href="'''Include File  Alignment.msf'''">Alignment.msf</a>.
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Here is an alignment of the HK022 cI repressor, the lambda cI repressor, the      P22 c2 repressor and the 434 cI repressor in msf format &lt;a href&#61;"'''Include File  Alignment.msf'''"&gt;Alignment.msf&lt;/a&gt;.
  
  
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<partinfo>BBa_C0050 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_C0050 SequenceAndFeatures</partinfo>
  
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
 
<partinfo>BBa_C0050 parameters</partinfo>
 
<partinfo>BBa_C0050 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: 5.9 ± 5.1% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>

Latest revision as of 22:38, 3 September 2020


cI repressor from phage HK022 (+LVA?)

Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.


Usage and Biology

Here is an alignment of the HK022 cI repressor, the lambda cI repressor, the P22 c2 repressor and the 434 cI repressor in msf format <a href="Include File Alignment.msf">Alignment.msf</a>.


Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 476
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 640



Functional Parameters: Austin_UTexas

BBa_C0050 parameters

Burden Imposed by this Part:

Burden Value: 5.9 ± 5.1%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.