Difference between revisions of "Part:BBa R1062"

 
 
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The lux cassette of V. fischeri contains a left and a right promoter. The      right promoter gives weak constitutive expression of downstream genes.This      expression is up-regulated by the action of the LuxR activator protein complexed      with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form      a complex with two molecules of the signalling compound homoserine lactone      (HSL). This complex binds to a palindromic site on the promoter, increasing      the rate of transcription.
 
The lux cassette of V. fischeri contains a left and a right promoter. The      right promoter gives weak constitutive expression of downstream genes.This      expression is up-regulated by the action of the LuxR activator protein complexed      with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form      a complex with two molecules of the signalling compound homoserine lactone      (HSL). This complex binds to a palindromic site on the promoter, increasing      the rate of transcription.
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===Usage and Biology===
 
===Usage and Biology===
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Medium strength promoter in the absense of LuxR/HSL.  May not be well matched with other '10 series' promoters. [jb 5/24/04]
 
Medium strength promoter in the absense of LuxR/HSL.  May not be well matched with other '10 series' promoters. [jb 5/24/04]
  
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<partinfo>BBa_R1062 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_R1062 SequenceAndFeatures</partinfo>
  
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
 
<partinfo>BBa_R1062 parameters</partinfo>
 
<partinfo>BBa_R1062 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: 3.6 ± 3.1% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>

Latest revision as of 19:27, 3 September 2020


Promoter, Standard (luxR and HSL regulated -- lux pR)

[Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].

Promoter activated by LuxR in concert with HSL

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.


Usage and Biology

Medium strength promoter in the absense of LuxR/HSL. May not be well matched with other '10 series' promoters. [jb 5/24/04]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_R1062 parameters

Burden Imposed by this Part:

Burden Value: 3.6 ± 3.1%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.