Difference between revisions of "Part:BBa I13001"
 
(7 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I13001 short</partinfo>
 
<partinfo>BBa_I13001 short</partinfo>
  
 
B0030. E0030. B0015
 
B0030. E0030. B0015
 +
 +
 +
== Experience ==
 +
Glasgow iGEM 2011
 +
 +
This part was found to express well when excited with fluorescence in the range of 515-565nm. See images below for expression testing:
 +
 +
[[Image:Gla_YFP_Results.jpg]]
 +
 +
----
 +
Shenzhen_SFLS (IGEM 2015)
 +
 +
We expressed this part in E.coli using pbad promoter and J23116 promoter.
 +
 +
From left to right: J23116/pbad(0μM L-Arabinose)/0.5μM/1.0μM/2.0μM(best in the 5 concentrations we usd)/4.0μM
 +
[[File:QQ图片20150210175650.jpg]] [[File:QQ图片20150210175655.jpg]]
 +
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 13: Line 30:
  
  
−
<!-- Uncomment this to enable Functional Parameter display
+
 
−
===Functional Parameters===
+
 
 +
==Functional Parameters: Austin_UTexas==
 +
<html>
 +
<body>
 
<partinfo>BBa_I13001 parameters</partinfo>
 
<partinfo>BBa_I13001 parameters</partinfo>
−
<!-- -->
+
<h3><center>Burden Imposed by this Part:</center></h3>
 +
<figure>
 +
<div class = "center">
 +
<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
 +
</div>
 +
<figcaption><center><b>Burden Value: 3.5 ± 5.7% </b></center></figcaption>
 +
</figure>
 +
<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
 +
<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
 +
<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
 +
</body>
 +
</html>

Latest revision as of 19:24, 3 September 2020

RBS B0030 w/ YFP (-LVA) TT

B0030. E0030. B0015


Experience

Glasgow iGEM 2011

This part was found to express well when excited with fluorescence in the range of 515-565nm. See images below for expression testing:

Gla YFP Results.jpg

Shenzhen_SFLS (IGEM 2015)

We expressed this part in E.coli using pbad promoter and J23116 promoter.

From left to right: J23116/pbad(0μM L-Arabinose)/0.5μM/1.0μM/2.0μM(best in the 5 concentrations we usd)/4.0μM QQ图片20150210175650.jpg QQ图片20150210175655.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_I13001 parameters

Burden Imposed by this Part:

Burden Value: 3.5 ± 5.7%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.