Difference between revisions of "Part:BBa K731520"
 
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This part is composed of [https://parts.igem.org/Part:BBa_K731500 K731500] and [https://parts.igem.org/Part:BBa_E0240 E0240] and allows the
 
This part is composed of [https://parts.igem.org/Part:BBa_K731500 K731500] and [https://parts.igem.org/Part:BBa_E0240 E0240] and allows the
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characterization of [https://parts.igem.org/Part:BBa_K731500 K731500]. It was successfully operated and characterized
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characterization of [https://parts.igem.org/Part:BBa_K731500 K731500]. It was successfully tested and characterized in both pSB4K5 and pSB1C3. Go to part K731500 for characterization results.
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in both pSB4K5 and pSB1C3.
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
 
<partinfo>BBa_K731520 parameters</partinfo>
 
<partinfo>BBa_K731520 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: 2.3 ± 3.4% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>

Latest revision as of 18:40, 3 September 2020

IPTG inducible GFPmut3b

This part is composed of K731500 and E0240 and allows the characterization of K731500. It was successfully tested and characterized in both pSB4K5 and pSB1C3. Go to part K731500 for characterization results.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1910



Functional Parameters: Austin_UTexas

BBa_K731520 parameters

Burden Imposed by this Part:

Burden Value: 2.3 ± 3.4%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.