Difference between revisions of "Part:BBa K819010"

 
 
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<partinfo>BBa_K819010 short</partinfo>
 
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cheZ ligated to a medium promoter. This part express CheZ protein mediumly and will induce medium mobility in delta cheZ E.coli strain.
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CheZ is a significant protein in <i>E.coli</i> chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.<br />
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CheZ was placed under a weak promoter [https://parts.igem.org/Part:BBa_J23113 BBa_J23113]. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.
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<img src="https://static.igem.org/mediawiki/parts/c/c2/Peking2012_part_CheZ_K819010.png" style="width:500px;margin-left:10%;"/>
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<p>Figure 1. <i>E.coli</i> was grown on semi-solid medium. (a) MG1655 strain with cheZ gene deleted; (b) CheZ gene under weak promoter BBa_J23112 ([https://parts.igem.org/Part:BBa_K819009 BBa_K819009]); (c) CheZ gene under medium promoter BBa_J23113 (this part); (d)wild type MG1655 strain;</p>
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<partinfo>BBa_K819010 parameters</partinfo>
 
<partinfo>BBa_K819010 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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<figcaption><center><b>Burden Value: 2.2 ± 3.4% </b></center></figcaption>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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Latest revision as of 18:34, 3 September 2020

Medium cheZ generator

CheZ is a significant protein in E.coli chemotaxis pathway. CheZ can prevent the cells from tumbling around and keep them moving. Deletion of CheZ or repression of CheZ expression can result in a non-motile phenotype.
CheZ was placed under a weak promoter BBa_J23113. This part expresses CheZ protein weakly and induces weak mobility in delta CheZ E.coli strain.

Figure 1. E.coli was grown on semi-solid medium. (a) MG1655 strain with cheZ gene deleted; (b) CheZ gene under weak promoter BBa_J23112 (BBa_K819009); (c) CheZ gene under medium promoter BBa_J23113 (this part); (d)wild type MG1655 strain;


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_K819010 parameters

Burden Imposed by this Part:

Burden Value: 2.2 ± 3.4%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.