Difference between revisions of "Part:BBa K523004"
 
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This is a version of <partinfo>BBa_K523002</partinfo> but with no RBS and no stop codon.
 
This is a version of <partinfo>BBa_K523002</partinfo> but with no RBS and no stop codon.
  
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There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site.
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There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site. There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for producing fusion proteins with Edinburgh 2011's BioSandwich assembly protocol (<nowiki>RFC 81</nowiki>).
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There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine.
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The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for Edinburgh 2011's BioSandwich assembly protocol.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
 
<partinfo>BBa_K523004 parameters</partinfo>
 
<partinfo>BBa_K523004 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: 1.7 ± 6.7% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>

Latest revision as of 20:36, 27 August 2020

bglX for BioSandwich

This is a version of BBa_K523002 but with no RBS and no stop codon.

There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site. There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for producing fusion proteins with Edinburgh 2011's BioSandwich assembly protocol (RFC 81).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1625
    Illegal AgeI site found at 1847
    Illegal AgeI site found at 2036
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_K523004 parameters

Burden Imposed by this Part:

Burden Value: 1.7 ± 6.7%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.