Difference between revisions of "Part:BBa B0013"
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__NOTOC__ | __NOTOC__ | ||
| + | {|width='80%' | ||
| + | |- | ||
| + | |width=35% valign='top'| | ||
| + | [[Image:Terminator.png]] | ||
| + | <partinfo>BBa_B0013 parameters</partinfo> | ||
| + | | width=50% valign='top' style='border: 1px solid black'| | ||
<partinfo>BBa_B0013 short</partinfo> | <partinfo>BBa_B0013 short</partinfo> | ||
| + | * Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional. | ||
| + | * But notice that the measurement indicates it is a strong promoter in the reverse direction. | ||
| + | |} | ||
| + | |||
| + | '''Secondary Structure''' | ||
| − | + | [[Image:Mfold-B0013-1.png]] | |
| − | + | <hr> | |
| − | + | '''Measurement''' | |
| + | * [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured] | ||
| − | |||
| − | |||
| − | + | ==Functional Parameters: Austin_UTexas== | |
| + | <html> | ||
| + | <body> | ||
<partinfo>BBa_B0013 parameters</partinfo> | <partinfo>BBa_B0013 parameters</partinfo> | ||
| + | <h3><center>Burden Imposed by this Part:</center></h3> | ||
| + | <figure> | ||
| + | <div class = "center"> | ||
| + | <center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center> | ||
| + | </div> | ||
| + | <figcaption><center><b>Burden Value: 0.6 ± 0.8% </b></center></figcaption> | ||
| + | </figure> | ||
| + | <p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the | ||
| + | <a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p> | ||
| + | <p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p> | ||
| + | </body> | ||
| + | </html> | ||
Latest revision as of 18:50, 27 August 2020
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TE from coliphage T7 (+/-)
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Secondary Structure
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.