Difference between revisions of "Part:BBa B0013"

 
(→‎Functional Parameters: Austin_UTexas)
 
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<SPAN style='position:absolute;font-size:90%;position:relative;top:-15px'>Designed by <partinfo>B0011 designer</partinfo></SPAN>
 
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<SPAN style='position:absolute;float:right;font-size:90%;position:relative;top:-15px'>Entered: <partinfo>B0011 entrydate</partinfo></SPAN>
 
 
__NOTOC__
 
__NOTOC__
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<partinfo>B0012 short</partinfo>
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{|width='80%'
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|-
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|width=35% valign='top'|
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[[Image:Terminator.png]]
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<partinfo>BBa_B0013 parameters</partinfo>
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| width=50% valign='top' style='border: 1px solid black'|
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<partinfo>BBa_B0013 short</partinfo>
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* Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
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* But notice that the measurement indicates it is a strong promoter in the reverse direction.
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|}
  
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Bidirectional transcriptional terminator consisting of a 22bp stem-loop
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'''Secondary Structure'''
  
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===Usage and Biology===
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[[Image:Mfold-B0013-1.png]]
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Appears to be a bidirectional terminator since it contains a string of T's on the direct strand after the stem loop and on the reverse strand before the
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stem loop. It is also found between two coding regions that point towards each other.
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<span class='h3bb'>Sequence and Features</span>
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<hr>
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<partinfo>B0012 SequenceAndFeatures</partinfo>
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'''Measurement'''
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
  
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===Functional Parameters===
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<partinfo>B0012 parameters</partinfo>
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
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<partinfo>BBa_B0013 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: 0.6 ± 0.8% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>

Latest revision as of 18:50, 27 August 2020

Terminator.png

biologyT7TE (+/-)
directionForward
forward_efficiency0.6[CC]
reversed_version591
reverse_efficiency-1.06[CC]

TE from coliphage T7 (+/-)

  • Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.
  • But notice that the measurement indicates it is a strong promoter in the reverse direction.

Secondary Structure

File:Mfold-B0013-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]


Functional Parameters: Austin_UTexas

BBa_B0013 parameters

Burden Imposed by this Part:

Burden Value: 0.6 ± 0.8%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.