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Contribution from uOttawa iGEM 2017
 
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<br>Author: Setti Belhouari  
 
<br>Author: Setti Belhouari  
 
<br>Biobrick <partinfo>BBa_B0031</partinfo> has been transformed into E. coli strain Mach1. Though five colonies have grown overnight, all of them tested negative for the emission of RFP through flow cytometry when compared to a zero control Mach1 strain. Perhaps expression expression of RFP may be induced using a stronger promoter.  
 
<br>Biobrick <partinfo>BBa_B0031</partinfo> has been transformed into E. coli strain Mach1. Though five colonies have grown overnight, all of them tested negative for the emission of RFP through flow cytometry when compared to a zero control Mach1 strain. Perhaps expression expression of RFP may be induced using a stronger promoter.  
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<partinfo>BBa_K516031 parameters</partinfo>
 
<partinfo>BBa_K516031 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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<figcaption><center><b>Burden Value: 0.2 ± 4.2% </b></center></figcaption>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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Latest revision as of 03:29, 26 August 2020

mRFP protein generator TERM+ RBS+(B0031)


Red fluorescent protein generator with BBa_B0031 low efficiency RBS (from Community Collection) and double terminator.
This construct is useful to measure the relative strength of promoters or the RBS efficiency in relationship with a promoter of interest.
To build a measurement device is only necessary assemble a standard biobrick promoter with this device (or another one of the collection: BBa_K516030 ; BBa_K516032)

Contribution from uOttawa iGEM 2017 T--uOttawa--neg ctrl o,2f,n 008.png T--uOttawa--Tube 001 001.png T--uOttawa--Tube 002 002.png T--uOttawa--Tube 003 003.png T--uOttawa--Tube 004 004.png T--uOttawa--Tube 005 005.png T--uOttawa--Tube 006 006.png T--uOttawa--Tube 007 009.png T--uOttawa--Tube 008 010.png T--uOttawa--Tube 009 011.png
Author: Setti Belhouari
Biobrick BBa_B0031 has been transformed into E. coli strain Mach1. Though five colonies have grown overnight, all of them tested negative for the emission of RFP through flow cytometry when compared to a zero control Mach1 strain. Perhaps expression expression of RFP may be induced using a stronger promoter.
Picture of plate.
CSV files.
Histograms.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 575
    Illegal AgeI site found at 687
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters: Austin_UTexas

BBa_K516031 parameters

Burden Imposed by this Part:

Burden Value: 0.2 ± 4.2%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.