Difference between revisions of "Part:BBa K516031"
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| − | Red fluorescent protein generator with <partinfo>BBa_B0031< | + | Red fluorescent protein generator with <partinfo>BBa_B0031</partinfo> low efficiency RBS (from Community Collection) and double terminator.<br> |
| − | This construct is useful | + | This construct is useful to measure the relative strength of promoters or |
the RBS efficiency in relationship with a promoter of interest.<br> | the RBS efficiency in relationship with a promoter of interest.<br> | ||
| − | To build a measurement device | + | To build a measurement device is only necessary assemble a standard biobrick promoter with this device |
(or another one of the collection: <partinfo>K516030</partinfo> ; <partinfo>K516032</partinfo>) | (or another one of the collection: <partinfo>K516030</partinfo> ; <partinfo>K516032</partinfo>) | ||
<br><br> | <br><br> | ||
| + | Contribution from uOttawa iGEM 2017 | ||
| + | [[File:T--uOttawa--neg ctrl o,2f,n_008.png]] | ||
| + | [[File:T--uOttawa--Tube_001_001.png]] | ||
| + | [[File:T--uOttawa--Tube_002_002.png]] | ||
| + | [[File:T--uOttawa--Tube_003_003.png]] | ||
| + | [[File:T--uOttawa--Tube_004_004.png]] | ||
| + | [[File:T--uOttawa--Tube_005_005.png]] | ||
| + | [[File:T--uOttawa--Tube_006_006.png]] | ||
| + | [[File:T--uOttawa--Tube_007_009.png]] | ||
| + | [[File:T--uOttawa--Tube_008_010.png]] | ||
| + | [[File:T--uOttawa--Tube_009_011.png]] | ||
| + | <br>Author: Setti Belhouari | ||
| + | <br>Biobrick <partinfo>BBa_B0031</partinfo> has been transformed into E. coli strain Mach1. Though five colonies have grown overnight, all of them tested negative for the emission of RFP through flow cytometry when compared to a zero control Mach1 strain. Perhaps expression expression of RFP may be induced using a stronger promoter. | ||
| + | <br>Picture of plate. | ||
| + | <br>CSV files. | ||
| + | <br>Histograms. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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| − | + | ||
| − | + | ==Functional Parameters: Austin_UTexas== | |
| + | <html> | ||
| + | <body> | ||
<partinfo>BBa_K516031 parameters</partinfo> | <partinfo>BBa_K516031 parameters</partinfo> | ||
| − | < | + | <h3><center>Burden Imposed by this Part:</center></h3> |
| + | <figure> | ||
| + | <div class = "center"> | ||
| + | <center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center> | ||
| + | </div> | ||
| + | <figcaption><center><b>Burden Value: 0.2 ± 4.2% </b></center></figcaption> | ||
| + | </figure> | ||
| + | <p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the | ||
| + | <a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p> | ||
| + | <p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p> | ||
| + | </body> | ||
| + | </html> | ||
Latest revision as of 03:29, 26 August 2020
mRFP protein generator TERM+ RBS+(B0031)
Red fluorescent protein generator with BBa_B0031 low efficiency RBS (from Community Collection) and double terminator.
This construct is useful to measure the relative strength of promoters or
the RBS efficiency in relationship with a promoter of interest.
To build a measurement device is only necessary assemble a standard biobrick promoter with this device
(or another one of the collection: BBa_K516030 ; BBa_K516032)
Contribution from uOttawa iGEM 2017
Author: Setti Belhouari
Biobrick BBa_B0031 has been transformed into E. coli strain Mach1. Though five colonies have grown overnight, all of them tested negative for the emission of RFP through flow cytometry when compared to a zero control Mach1 strain. Perhaps expression expression of RFP may be induced using a stronger promoter.
Picture of plate.
CSV files.
Histograms.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 575
Illegal AgeI site found at 687 - 1000COMPATIBLE WITH RFC[1000]
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.