Difference between revisions of "Part:BBa R0063"

 
 
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<partinfo>BBa_R0063 short</partinfo>
 
<partinfo>BBa_R0063 short</partinfo>
  
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The lux cassette of V. fischeri contains a left and a right promoter. The      left promoter gives weak constitutive expression of downstream genes.This      expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules      of LuxR protein form a complex with two molecules the signalling compound      homoserine lactone (HSL). This complex binds to a palindromic site on the      promoter, increasing the rate of transcription from Pr, repressing transcription      from Pl</p>
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The lux cassette of V. fischeri contains a left and a right promoter. The      left promoter gives weak constitutive expression of downstream genes.This      expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules      of LuxR protein form a complex with two molecules the signalling compound      homoserine lactone (HSL). This complex binds to a palindromic site on the      promoter, increasing the rate of transcription from Pr, repressing transcription      from Pl
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===Usage and Biology===
 
===Usage and Biology===
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Medium strength promoter in the absence of LuxR/HSL. [jb, 5/24/04]
 
Medium strength promoter in the absence of LuxR/HSL. [jb, 5/24/04]
  
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<partinfo>BBa_R0063 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_R0063 SequenceAndFeatures</partinfo>
  
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
 
<partinfo>BBa_R0063 parameters</partinfo>
 
<partinfo>BBa_R0063 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: -2.3 ± 4.1% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>

Latest revision as of 02:27, 26 August 2020


Promoter (luxR & HSL regulated -- lux pL)

The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl


Usage and Biology

Medium strength promoter in the absence of LuxR/HSL. [jb, 5/24/04]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_R0063 parameters

Burden Imposed by this Part:

Burden Value: -2.3 ± 4.1%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.