Difference between revisions of "Part:BBa K523003"

 
 
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<partinfo>BBa_K523003 short</partinfo>
 
<partinfo>BBa_K523003 short</partinfo>
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This is a version of <partinfo>BBa_K523001</partinfo> but with no RBS and no stop codon.
 
This is a version of <partinfo>BBa_K523001</partinfo> but with no RBS and no stop codon.
  
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There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. This part is intended to be used with Edinburgh 2011's "BioSandwich" protocol, in which a BglII site before the coding sequence, and a SpeI site after the coding sequence (i.e. the one in the suffix) are used for assembly.
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There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site. There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for producing fusion proteins with Edinburgh 2011's BioSandwich assembly protocol (<nowiki>RFC 81</nowiki>).
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K523003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K523003 SequenceAndFeatures</partinfo>
  
  
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<!-- Uncomment this to enable Functional Parameter display
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==Functional Parameters: Austin_UTexas==
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===Functional Parameters===
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<html>
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<body>
 
<partinfo>BBa_K523003 parameters</partinfo>
 
<partinfo>BBa_K523003 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: -1.0 ± 2.8% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>

Latest revision as of 04:10, 25 August 2020

malS for BioSandwich

This is a version of BBa_K523001 but with no RBS and no stop codon.

There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site. There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for producing fusion proteins with Edinburgh 2011's BioSandwich assembly protocol (RFC 81).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1204
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
    Illegal AgeI site found at 1505
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters: Austin_UTexas

BBa_K523003 parameters

Burden Imposed by this Part:

Burden Value: -1.0 ± 2.8%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.