Difference between revisions of "Part:BBa K3183004"
(→‎Reference)
 
(One intermediate revision by the same user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3183004 short</partinfo>
 
<partinfo>BBa_K3183004 short</partinfo>
  
−
Quorum sensing transcription factor from <i>C. difficile</i>.  It is one of the members of the two-component detection system, adapted from <i>C. difficile</i>, that is bound by AgrC2 in response to Autoinducing peptide (AIP), which phosphorylates it. The released phosphorylated AgrA2 dimerizes with itself and becomes active. Upon activation, it binds to AgrPromoter (<partinfo>BBa_K3183003</partinfo>) which results in upregulation of transcription. This gene is located in C. difficile CD196 genome.
+
Quorum sensing transcription factor from <i>C. difficile</i>.  It is a member of the two-component detection system, adapted from <i>C. difficile</i>, that binds and gets phosphorylated by AgrC2 in response to interaction with the Autoinducing peptide (AIP). The released and phosphorylated AgrA2 dimerizes with itself and becomes active. Upon activation, it binds to AgrPromoter (<partinfo>BBa_K3183003</partinfo>) which results in upregulation of transcription.<sup>1</sup> This gene is located in <i>C. difficile</i> CD196 genome.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 20: Line 20:
 
===Use by Team Oxford 2019===
 
===Use by Team Oxford 2019===
 
This transcription factor is part of our detection system in <i>L. reuteri</i>. The composite parts containing this AgrA2 protein are <partinfo>BBa_K3183102</partinfo>, and <partinfo>BBa_K3183300</partinfo>.
 
This transcription factor is part of our detection system in <i>L. reuteri</i>. The composite parts containing this AgrA2 protein are <partinfo>BBa_K3183102</partinfo>, and <partinfo>BBa_K3183300</partinfo>.
 +
 +
===Reference===
 +
1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.

Latest revision as of 22:17, 21 October 2019


AgrA2 Quorum Sensing Transcription Factor; C. difficile

Quorum sensing transcription factor from C. difficile. It is a member of the two-component detection system, adapted from C. difficile, that binds and gets phosphorylated by AgrC2 in response to interaction with the Autoinducing peptide (AIP). The released and phosphorylated AgrA2 dimerizes with itself and becomes active. Upon activation, it binds to AgrPromoter (BBa_K3183003) which results in upregulation of transcription.1 This gene is located in C. difficile CD196 genome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Use by Team Oxford 2019

This transcription factor is part of our detection system in L. reuteri. The composite parts containing this AgrA2 protein are BBa_K3183102, and BBa_K3183300.

Reference

1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.