Difference between revisions of "Part:BBa K103020:Experience"

Revision as of 13:14, 26 October 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K103020

'Hunter and prey' selection system

This part was used as core of prey generator device. It was fused with:

protein A BBa_K103003. Resulting fusion was named His_omega_A
protein Z_SPA-1 BBa_K103004. . Resulting fusion was named His_omega_Z

Both fusions were overexpressed in BL21 strain. Due to ability of protein Z to form aggregates His_omega_Z was purified simply by collecting post-sonication debris. His_omega_A was purified on NiNTA-agarose bead (elution peak in 150 mM imidazole). The both prey protein fusions were added to culture media and used in selection experiments. Details here and here.

User Reviews

UNIQ7b10c2050db7e24d-partinfo-00000000-QINU UNIQ7b10c2050db7e24d-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
@@ Line 5: / Line 4: @@
 ===Applications of BBa_K103020===
+==='Hunter and prey' selection system===
+This part was used as core of prey generator device. It was fused with:
+*protein A [[Part:BBa_K103003 | BBa_K103003]]. Resulting fusion was named His_omega_A
+*protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. . Resulting fusion was named His_omega_Z
+Both fusions were overexpressed in BL21 strain. Due to ability of protein Z to form aggregates His_omega_Z was purified simply by collecting post-sonication debris.
+His_omega_A was purified on NiNTA-agarose bead (elution peak in 150 mM imidazole).
+The both prey protein fusions were added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]].
 ===User Reviews===